Jul 17, 2023 · Real-time monitoring of microbial dynamics during fermentation is essential for wine quality control. This study developed a method that combines the fluorescent dye propidium monoazide (PMA) with CELL-qPCR, which can distinguish between dead and live microbes for Lactiplantibacillus plantarum. This method could detect the quantity of microbes efficiently and rapidly without DNA extraction
Jul 17, 2019 · When the fermenter with hollow fiber membrane is used for the submerged fermentation of L. lactis subsp. lactis, the ultrafiltration system is continuously operated at fermentation time of 5–12 h, and the whey, lactose, and yeast extracts are supplemented; at the fermentation of 12–30 h, the medium was removed/recirculated every 30 min for
Jul 26, 2020 · Tower fermentation. It was defined by Greenshields and co-workers. It is an elongated non-mechanically type of fermenters. There is unidirectional gases flow throughout the bioreactors. Tower fermenters are of following types: Bubble columns; Vertical tower beer fermenters; Multistage fermenters; Bubble up fermenters
May 9, 2024 · DO saturation in the fermentation broth is a crucial response variable, directly correlating with overflow metabolism and oxygen consumption. Utilizing reliable probes for regular and precise DO measurements offers a practical and effective means of controlling the feed rate in fermentation processes.
Fermentation of a Variety of Carbohydrates. Bacteria, depending on the species, can ferment different carbohydrates. Although glycolysis (the pathway leading to fermentation) begins with glucose, some bacteria have the enzymes needed for additional chemical reactions to convert other monosaccharides (e.g. fructose and mannose) as well as disaccharides (e.g. lactose and sucrose) so they can
Nov 18, 2021 · Kombucha fermentation comprises three main types of fermentation (i.e., alcoholic, lactic, and acetic) due to the presence of different types of bacteria and yeast co-existing in the medium and responsible for different stages of fermentation; the process is initiated by osmotolerant microbes, and acid-tolerant bacteria prevail and dominate .
Jan 1, 2022 · This chapter gives an overview of fermentation process design, industrial fermentation processes, and current trends in food fermentation and the impact of advances in synthetic biology on fermentation and its implication to sustainable manufacture of food ingredients.
Sep 8, 2011 · The B. subtilis mannose operon consists of three genes, manP, manA, and yjdF (). manP encodes the p hospho t ransferase s ystem (PTS) mannose-specific enzyme IIBCA which belongs to the Fru-permease family (), and manA encodes a mannose 6-phosphate isomerase which converts the incoming mannose 6-phosphate to fructose 6-phosphate that subsequently enters glycolysis.
Aug 10, 2022 · Fermentation media are used to differentiate organisms based on their ability to ferment carbohydrates incorporated into the basal medium. Phenol Red Broth Medium with various added carbohydrates serves as a differential medium by aiding in differentiation of various species and genera by their ability to ferment the specific carbohydrate, with the production of acid or acid and gas.
A novel technically compliant expression system was developed for heterologous protein production in Bacillus subtilis with the aim of increasing product yields at the same time as decreasing production costs. Standard systems involve the positively regulated manP promoter of the mannose operon, whi
Notwithstanding the reliable value of the spontaneous fermentation to stabilize and preserve raw vegetables and fruits, a number of factors are in favour of using selected starters. Two main options may be pursued for the controlled lactic acid fermentation of vegetables and fruits: the use of commercial/allochthonous and the use of
History of Microbiology. W.C. Summers, in Encyclopedia of Microbiology (Third Edition), 2009 Microbial Physiology. With the recognition of the role of microbes in ancient processes such as fermentations and other types of food processing (cheese, bread, soy sauce, and silage), a better understanding of these uses of microbes and their by-products was possible.
A Fermentor is a device used to accomplish the fermentation process by using microorganisms, and for this reason, it is also called “Biofermentor or Bioreactor“.It is equipped with all the elements that are necessary to carry out the commercial production of substances like antibiotics, enzymes, beverages etc. in many industries.
Apr 27, 2017 · The main function of fermentation is to convert NADH back into the coenzyme NAD + so that it can be used again for glycolysis. During fermentation, an organic electron acceptor (such as pyruvate or acetaldehyde) reacts with NADH to form NAD +, generating products such as carbon dioxide and ethanol (ethanol fermentation) or lactate (lactic acid fermentation) in the process.